primary antibodies against human leukocyte antigen–antigen d related (ln3)  (Thermo Fisher)

Journal: Clinical and Experimental Immunology

Article Title: High activation and skewed T cell differentiation are associated with low IL‐17A levels in a hu‐PBL‐NSG‐SGM3 mouse model of HIV infection

doi: 10.1111/cei.13416

Figure Lengend Snippet: Higher T cell activation in non‐obese diabetic/severe combined immunodeficient/interleukin‐2 (NOD/SCID/IL‐2) receptor ‐chainnull (NSG) mice transgenic for human stem cell factor (SCF) (NSG‐SGM3) mice humanized with human peripheral blood mononuclear cells (PBMC) (hu‐PBL‐NSG‐SGM3) HIV+ mice. (a) Expression of human leukocyte antigen D‐related (HLA‐DR) and CD38 and programmed cell death 1 (PD‐1) and inducible T cell co‐stimulator (ICOS) in CD8+ and CD4+ T cells from the seronegative and HIV‐infected donors. (b,c) Representative contour plots of the expression of HLA‐DR and CD38 and PD‐1 and ICOS in circulating CD8+ (b) and CD4+ T cells (c) from hu‐PBL‐NSG‐SGM3 HIV−, HIV+ untreated and HIV+ anti‐retroviral therapy (ART) mice at week 7 post‐engraftment. (d,e) Frequencies of HLA‐DR+CD38+, PD‐1+ and ICOS+CD8+ (d) and CD4+ T cells (e) from hu‐PBL‐NSG‐SGM3 HIV− (n = 10), HIV+ untreated (n = 3) and HIV+ ART (n = 9) mice throughout monitoring time. (c–e) *P ≤ 0·03 versus the other mouse groups, Kruskal–Wallis test. (d,e) The gray box indicates ART administration.

Article Snippet: For phenotypical analyses, whole blood samples were incubated for 30 min at room temperature with optimized doses of the following anti‐human antibodies: CD3‐fluorescein isothiocyanate (FITC) (clone HIT3a; BD Biosciences, San Jose, CA, USA), CD4‐BV421 (clone RPA‐T4; BD Biosciences), CD8‐Alexa Fluor (AF) 700 (clone OKT8; Thermo Fisher, Waltham, MA, USA), human leukocyte antigen D‐related (HLA‐DR)‐antigen‐presenting cells (APC)‐eFluor 780 (clone LN3; Thermo Fisher), CD38‐phycoerythrin (PE) eFluor 610 (clone HIT2; Thermo Fisher), programmed cell death 1 (PD‐1)‐BV510 (clone EH12.1; BD), inducible T cell co‐stimulator (ICOS) AF647 (clone DX29, BD), CCR7‐PE (clone 3D12; Thermo Fisher) and CD45RA‐PE cyanin 7 (Cy7) (clone HI100; Biolegend, San Diego, CA, USA).

Techniques: Activation Assay, Transgenic Assay, Expressing, Infection

Journal: Frontiers in Aging Neuroscience

Article Title: Activated Microglia in Cortical White Matter Across Cognitive Aging Trajectories

doi: 10.3389/fnagi.2019.00094

Figure Lengend Snippet: Human leukocyte antigen-D related (HLA-DR)-positive activated microglia are shown in the white matter of the inferior frontal gyrus (IFG) in (A) SuperAger (Case 17), (B) Young (Case 2), and (C) Normal Old (Case 9). Microglia immunoreactive to Iba1 are shown in corpus callosum (CC) in (D) SuperAger (Case 16), (E) Young (Case 2), and (F) Normal Old (Case 10). Photomicrographs were acquired at 20× magnification. Scale bar = 100 μm.

Article Snippet: Activated microglia were visualized immunohistochemically using an antibody against the human leukocyte antigen-D related (HLA-DR) protein (mouse monoclonal; Dako; 1/1000).

Techniques:

Journal: Frontiers in Aging Neuroscience

Article Title: Activated Microglia in Cortical White Matter Across Cognitive Aging Trajectories

doi: 10.3389/fnagi.2019.00094

Figure Lengend Snippet: (A) Moderate white matter hyperintensities (WMHs) are observed in periventricular regions on T2 FLAIR MRI from Case #17, a SuperAger. (B) Representative HLA-DR staining in a whole-hemisphere coronal section from Case #17 at the level of the WMH shown in (A) , demarcated by the gray dotted circle. In comparison to regions of white matter without observed WMHs (C) , regions with observed white matter hyperintensities (D) displayed less HLA-DR-immunopositivity, which was reflected by smaller, less dense populations of activated microglia. Photomicrographs were acquired at 20× magnification.

Article Snippet: Activated microglia were visualized immunohistochemically using an antibody against the human leukocyte antigen-D related (HLA-DR) protein (mouse monoclonal; Dako; 1/1000).

Techniques: Staining